Monitoring airway mucus flow and ciliary activity with optical coherence tomography
نویسندگان
چکیده
Muco-ciliary transport in the human airway is a crucial defense mechanism for removing inhaled pathogens. Optical coherence tomography (OCT) is well-suited to monitor functional dynamics of cilia and mucus on the airway epithelium. Here we demonstrate several OCT-based methods upon an actively transporting in vitro bronchial epithelial model and ex vivo mouse trachea. We show quantitative flow imaging of optically turbid mucus, semi-quantitative analysis of the ciliary beat frequency, and functional imaging of the periciliary layer. These may translate to clinical methods for endoscopic monitoring of muco-ciliary transport in diseases such as cystic fibrosis and chronic obstructive pulmonary disease (COPD).
منابع مشابه
Method for Quantitative Study of Airway Functional Microanatomy Using Micro-Optical Coherence Tomography
We demonstrate the use of a high resolution form of optical coherence tomography, termed micro-OCT (μOCT), for investigating the functional microanatomy of airway epithelia. μOCT captures several key parameters governing the function of the airway surface (airway surface liquid depth, periciliary liquid depth, ciliary function including beat frequency, and mucociliary transport rate) from the s...
متن کاملEffect of Cilia Beat Frequency on Muco-ciliary Clearance
Background: The airway surface liquid (ASL), which is a ï‚uid layer coating the interior epithelial surface of the bronchi and bronchiolesis, plays an important defensive role against foreign particles and chemicals entering lungs.Objective: Numerical investigation has been employed to solve two-layer model consisting of mucus layer as a viscoelastic fluid and periciliary liquid layer as a New...
متن کاملMicrofluidic characterization of cilia-driven fluid flow using optical coherence tomography-based particle tracking velocimetry
Motile cilia are cellular organelles that generate directional fluid flow across various epithelial surfaces including the embryonic node and respiratory mucosa. The proper functioning of cilia is necessary for normal embryo development and, for the respiratory system, the clearance of mucus and potentially harmful particulate matter. Here we show that optical coherence tomography (OCT) is well...
متن کاملNovel Endoscopes for Microscopic Assessment of Airway Clearance using Micro - Optical Coherence Tomography by Carolin
The health of the human respiratory system depends critically on airway clearance via motile hair-like structures (cilia), which transport and eliminate unwanted particles trapped within mucus. Impairment of mucociliary clearance (MCC) can lead to lifethreatening airway narrowing and lung infections, and is a major cause of morbidity and mortality in patients with cystic fibrosis, primary cilia...
متن کاملDevelopment of an airway mucus defect in the cystic fibrosis rat.
The mechanisms underlying the development and natural progression of the airway mucus defect in cystic fibrosis (CF) remain largely unclear. New animal models of CF, coupled with imaging using micro-optical coherence tomography, can lead to insights regarding these questions. The Cftr-/- (KO) rat allows for longitudinal examination of the development and progression of airway mucus abnormalitie...
متن کامل